Biological models
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Biological models that I have experience to work with are:
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Mammalian cells cultured in vitro (research internships, doctoral thesis, postdoctoral research;this is my major biological model)
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Mouse (doctoral thesis, postdoctoral research)
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Yeast (Master thesis)
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Bacteria (Master thesis and research internships)
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Each biological model has its advantages as well as limitations. Bacteria and yeast divide very quickly and are commonly used as a tool in genetics. Mouse models may recapitulate many aspects of human diseases, but still those models are complex and mice have different diet and immune system when compared to humans. Mammalian cells represent a research model that can be easily manipulated in vitro to learn about cellular and molecular mechanisms of human diseases. My the most commonly used biological model has been the mammalian cells, including patient-derived cells.
I have experience in culturing cell lines as well as establishing primary cell cultures. I am familiar with cell cultures from various species e.g. human, mouse and dog. I can culture healthy cells as well as cancer cells. In case of tumor cells, I cultured both cancer cells from solid tumors as well as tumor cells cultured in suspension.
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I started my cell culture adventure with mammalian cells firstly in the form of standard two dimensional (2D) cell culture, in which cells are attached to plastic. Recently, I have been working a lot on three-dimensional (3D) cell culture models, performing both epithelial cell monocultures and epithelium-stroma co-cultures.
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