Join me!
1. Collaboration partners
If you are interested in exchanging research ideas, applying together for grants, writing papers together and discussing recently published papers in the field of: stem cell biology, cancer, the tissue microenvironment and personalised medicine, and working together towards shared goals, please contact me: agnieszka.pastula@wp.pl
2. Visiting scientists, summer students, undergraduate students and graduate students
I have >15 years of full-time experience in research, including voluntary research activities, extracurricular research intrernships, research during MS and PhD, postdoctoral trainings and working as a PI. I have extensive international experience, I was working in Poland, Germany, Czech Republic, the Netherlands and in the USA. I have a very solid background in molecular and cell biology, with expertise in studying the tissue microenvironment.
If you are very motivated and talented local or international student, preferably willing to apply for a scholarship/ fellowship, I am happy to share my knowledge, work with you and train you:
Cellular gastroenterology
- small intestinal organoid culture
- different organoid assays: organoid spheroid assay, organoid diameter, organoid survival, single cell-derived organoids
- colon tumor organoid culture e.g. derived colon tumors from Dro1+/+;ApcMin/+ and Dro1-/-;ApcMin/+ mice
- human-derived Barrett's esophagus organoid culture
- intestinal fibroblast isolation and culture
- Barrett's esophagus fibroblast isolation and culture
- various epithelial-stromal co-culture systems to model the gastrointestinal tissue microenvironment e.g. indirect co-culture of intestinal fibroblasts and intestinal crypts and organotypic (multi-layered, air-liquid interface) cultures of gastrointestinal epithelium and the stroma
Experimental urology
- dissection of the prostate
- isolation of the urogenital sinus from E15.5 and E16.5 mouse embryos
- urogenital sinus explant culture
- isolation and culturing of primary urogenital mesenchymal cells
- isolation and culture of stromal cells derived from human prostatectomy samples
- single cell analysis of stromal cell subtypes in the prostate (software: 10x genomics)
Other methods
- 2D and 3D culture of Madin Darby Canine Kidney Cell (MDCK) cell line
- induction of epithelial-mesenchymal transition in MDCK cells
- culturing HeLa Flp-In cell line expressing GFP-GRAF and mCherry-Rab8
- dextran uptake assay using pancreatic tumor organoids
- RNA isolation, real-time PCR
- RNA microarray, DAVID bioinformatics
- western blotting
- culturing Saccharomyces cerevisiae
- yeast growth assay (spot testing S. cerevisiae)
- culturing Escherichia coli
- molecular cloning
- mammalian cell transfection
- trogocytosis assay
- isolation of murine embryonic fibroblasts (MEFs)
- isolation of bone marrow-derived cells
- cryosectioning
- sectioning of paraffin-embedded tissue
- immunofluorescent staining of PFA-fixed frozen tissue slices and PFA-fixed cells seeded into chamber slides
- immunohistochemistry stainings
- basics of mouse husbandry and breeding
- setting up timed pregnant mice
- confocal microscopy (Leica TCS SP5 & Zeiss 710)
If you are interested in any form of collaboration or working together, please send an e-mail to Dr. Pastula: agnieszka.pastula@wp.pl